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Related Experiment Videos

An improved turbidimetric method for plasma fibrinogen

D B Cannarozzi, S C Wardlaw, P McPhedran

    The American Journal of Medical Technology
    |March 1, 1977
    PubMed
    Summary

    This study validates Ellis and Stransky's turbidimetric method for plasma fibrinogen determination. The accurate, sensitive, and cost-effective assay provides rapid results, unaffected by common interfering substances.

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    Area of Science:

    • Clinical Chemistry
    • Hematology

    Background:

    • Plasma fibrinogen is a critical biomarker for hemostasis.
    • Accurate and efficient fibrinogen measurement is essential for clinical diagnostics.

    Purpose of the Study:

    • To present experience with Ellis and Stransky's turbidimetric technique for plasma fibrinogen determination.
    • To validate the turbidimetric method against a reference standard.

    Main Methods:

    • Standardization of the turbidimetric assay using a plasma pool calibrated by the weighed clot technique.
    • Evaluation of assay accuracy, sensitivity, specificity, and reproducibility.
    • Assessment of interference from lipids, bilirubin, fibrin split products, lymphangiogram dye, and heparin.

    Main Results:

    • The turbidimetric method demonstrated high accuracy, sensitivity (down to 25 mg/dl), specificity, and reproducibility (CV of 3.4%).
    • Results were unaffected by common interfering substances.
    • The assay is convenient, inexpensive (reagent cost < $0.01/test), and provides results for 10 samples within 30 minutes.

    Conclusions:

    • The Ellis and Stransky turbidimetric technique is a reliable, efficient, and cost-effective method for plasma fibrinogen measurement.
    • This method is suitable for routine clinical laboratory use due to its speed and robustness.

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