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Factors affecting T3 and T4 proficiency testing

I L Hubert, W Shaw, F W Spierto

The American Journal of Medical Technology

|April 1, 1977

Summary

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A new normalization method simplifies thyroid hormone testing performance evaluation. Studies reveal that sample stability issues and fatty acid interference affect T3 uptake and T4 measurements, impacting accuracy in proficiency testing programs.

Area of Science:

Clinical Chemistry
Endocrinology
Laboratory Medicine

Background:

Proficiency testing (PT) programs face challenges in performance evaluation and sample stability for thyroid hormone assays.
Varied units in T3 uptake methodologies complicate accurate performance assessment.
Existing methods for T4 measurement are susceptible to interference from fatty acids.

Purpose of the Study:

To develop a normalization method for T3 uptake performance evaluation.
To investigate sample stability for T3 uptake and T4 assays at room temperature.
To identify and characterize interfering substances in T4 measurement methods.

Main Methods:

A novel normalization method was developed for T3 uptake assays.
Sample stability was assessed over time at room temperature for both T3 uptake and T4.

Related Experiment Videos

Fatty acid interference was evaluated in T4 competitive protein binding (CPB) and radioimmunoassay (RIA) methods using varying concentrations and chain lengths.

Main Results:

The developed normalization method facilitates T3 uptake performance evaluation.
T3 uptake sample values increase after approximately seven days of storage at room temperature.
T4 content in pooled serum increases after about one week at room temperature.
Fatty acids interfere with both T4 CPB and RIA methods, with interference increasing as carbon chain length decreases and unsaturation increases.
Fatty acid interference is more pronounced in the CPB method compared to RIA.
Fatty acids are likely responsible for the observed increase in T3 uptake values.

Conclusions:

A normalization method can improve T3 uptake performance evaluation in PT programs.
Room temperature storage negatively impacts the stability of T3 uptake and T4 samples.
Fatty acids are significant interfering substances in T4 assays, particularly affecting CPB methods, and may also affect T3 uptake results.