Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple generational bovine embryo cloning

S L Stice1, C L Keefer

  • 1ABS Specialty Genetics, DeForest, Wisconsin 53532.

Biology of Reproduction
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time within reproductive season, but not age or inbreeding coefficient, affects seminal and sperm quality in the whooping crane (Grus americana).

Reproduction, fertility, and development·2015
Same author

Molecular markers of spermatogonial stem cells in the domestic cat.

Reproduction in domestic animals = Zuchthygiene·2013
Same author

Livestock induced pluripotent stem cells.

Reproduction in domestic animals = Zuchthygiene·2012
Same author

Ion channels and ionotropic receptors in human embryonic stem cell derived neural progenitors.

Neuroscience·2011
Same author

KIT ligand and bone morphogenetic protein signaling enhances human embryonic stem cell to germ-like cell differentiation.

Human reproduction (Oxford, England)·2009
Same author

Lessons learned from nuclear transfer (cloning).

Theriogenology·2007
Same journal

Reduced Circulating Polyamines in Polyendocrine Metabolic Ovarian Syndrome (PMOS) Patients and the Impact of Putrescine on Ovarian Function and Fertility in a Murine PMOS Model.

Biology of reproduction·2026
Same journal

DEVELOPMENTAL PROGRAMMING IN DOGS.

Biology of reproduction·2026
Same journal

Meiosis-Specific Cohesin in Mammalian Germ Cells.

Biology of reproduction·2026
Same journal

The Research of Lactylation in the Field of Reproduction.

Biology of reproduction·2026
Same journal

Mitochondria as Integrative Regulators of Ferroptosis in the Female Reproductive System.

Biology of reproduction·2026
Same journal

Retraction and replacement of: Navigation of Nanos germ cell specification factor to germ granules-posttranscriptional regulation hubs-across species.

Biology of reproduction·2026
See all related articles

Repeated nuclear transfer (cloning) can produce multiple generations of genetically identical animals. However, efficiency and developmental rates vary significantly across embryo generations, impacting cloning success.

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Animal cloning

Background:

  • Nuclear transfer (NT) allows for the production of genetically identical mammalian offspring.
  • Understanding efficiency across multiple generations is crucial for optimizing cloning techniques.
  • Embryo stage and donor generation impact NT success rates.

Purpose of the Study:

  • To compare nuclear transfer procedure efficiencies and developmental rates across different embryo stages and generations.
  • To assess the impact of in vitro culture duration on cell number and subsequent NT efficiency.
  • To evaluate fusion and developmental rates of clones from parent and subsequent generations.

Main Methods:

  • Nuclear transfer was performed using embryos from parent and successive generations (first, second, third).

Related Experiment Videos

  • Embryos were cultured for 4 or 5 days in the sheep oviduct prior to NT.
  • Fusion rates of donor cells and developmental rates of resulting embryos were recorded and analyzed.

    • Main Results:

    • 54 genetically identical embryos developed to the morula stage from a single parent embryo.
    • Five-day cultured clones had more cells but lower fusion rates compared to 4-day cultured clones.
    • Fusion rates declined with each generation of clones, and second-generation donors showed the lowest developmental rates.

    Conclusions:

    • Nuclear transfer efficiency and developmental potential vary significantly between embryo generations.
    • Optimizing culture duration and understanding nuclear reprogramming are key to improving cloning efficiency.
    • Further research may enable the production of unlimited genetically identical animals.