Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human epithelial ovarian cancer allelotype

W Cliby1, S Ritland, L Hartmann

  • 1Department of Gynecologic Oncology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.

Cancer Research
|May 15, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calprotectin as Diagnostic Marker for Hepatic Encephalopathy and Spontaneous Bacterial Peritonitis in Cirrhosis.

Clinical laboratory·2020
Same author

Colectomy with ileostomy for severe ulcerative colitis-postoperative complications and risk factors.

International journal of colorectal disease·2019
Same author

Exploiting Oligo(amido amine) Backbones for the Multivalent Presentation of Coiled-Coil Peptides.

Biomacromolecules·2015
Same author

Fluorescence in situ hybridization identifies high risk Barrett's patients likely to develop esophageal adenocarcinoma.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2015
Same author

FISHing for pancreatobiliary tract malignancy in endoscopic brushings enhances the sensitivity of routine cytology.

Cytopathology : official journal of the British Society for Clinical Cytology·2014
Same author

Characterization of a cDNA encoding ricin E, a hybrid ricin-Ricinus communis agglutinin gene from the castor plant Ricinus communis.

Plant molecular biology·2013
Same journal

Targeting Cystine Addiction Suppresses Breast Cancer Lung Metastasis.

Cancer research·2026
Same journal

SEPTIN7 Enhances Tumorgenesis and Therapeutic Resistance in Hepatocellular Carcinoma by Promoting FGFR4 Stabilization and Recycling.

Cancer research·2026
Same journal

TLR9 Agonists Potentiate Adoptive T Cell Therapy in Cancer through a B Cell-CD2 Costimulatory Axis.

Cancer research·2026
Same journal

CDK2 Inhibition Exerts RB-Independent Antitumor Activity in CDK4/6 Inhibitor-Resistant HR+/HER2- Breast Cancer.

Cancer research·2026
Same journal

A Clinically Integrated Pediatric Patient-Derived Xenograft Program Enables Evaluation of Cohort and Patient-Specific Biology and Therapeutic Strategies.

Cancer research·2026
Same journal

Editor's Note: Heterodimerization of Insulin-like Growth Factor Receptor/Epidermal Growth Factor Receptor and Induction of Survivin Expression Counteract the Antitumor Action of Erlotinib.

Cancer research·2026
See all related articles

This study identified key chromosomal regions associated with human epithelial ovarian cancer by analyzing loss of heterozygosity (LOH) in tumors. High-grade tumors showed significantly more LOH, particularly on chromosomes 6, 13, and 17, indicating potential tumor suppressor gene locations.

Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • Human epithelial ovarian cancer (EOC) is a significant health concern.
  • Identifying tumor suppressor genes is crucial for understanding EOC development.
  • Loss of heterozygosity (LOH) is a common genetic alteration in cancer.

Purpose of the Study:

  • To pinpoint specific chromosomes and chromosomal regions harboring tumor suppressor genes relevant to human epithelial ovarian cancer.
  • To investigate the correlation between LOH frequency and tumor grade in EOC.

Main Methods:

  • Loss of heterozygosity (LOH) analysis was performed on 37 primary epithelial ovarian tumors.
  • Seventy polymorphic markers were used to examine all chromosome arms (excluding acrocentric arms).
  • Detailed analysis of chromosomes 6, 13, and 17 was conducted to localize deletion regions.

Related Experiment Videos

Main Results:

  • High frequencies of LOH were observed on multiple chromosome arms, including 5q, 6p, 6q, 9q, 13q, 17p, 17q, and 22q.
  • Statistically significant differences in LOH were found between low-grade and high-grade tumors, especially on chromosomes 6p, 13q, 17p, and 17q.
  • High-grade tumors exhibited a higher average fractional allelic loss (0.40) compared to low-grade tumors (0.17).
  • Regional deletions on chromosome 6, specifically distal 6q and near the centromere proximal to HLA, were implicated in EOC development.

Conclusions:

  • Specific chromosomal regions, including parts of chromosomes 6, 13, and 17, are critical in human epithelial ovarian cancer development.
  • Tumor suppressor genes located in these regions likely play a significant role in EOC pathogenesis.
  • The frequency of LOH and fractional allelic loss increases with tumor grade, suggesting a role in tumor progression.