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Detailed characterization of a human 8q24.1 microdissection library and generation of "sequence-tagged sites"
B La Pillo1, S Karpinski, H J Lüdecke
1Institut für Humangenetik, Universitätsklinikum Essen, FRG.
Cytogenetics and Cell Genetics
|January 1, 1993
Abstract:
A total of 533 clones from a human 8q24.1 microdissection library was analyzed by automatic DNA sequencing. Three hundred and thirty-seven different insert sequences were found. The insert size ranged from 45 to 376 bp (mean, 170 bp). Eighty-six percent (291/337) of these sequences were free of repetitive DNA. Each of 19 clones tested was successfully translated into a sequence-tagged site. We conclude that the microdissection library is a rich source of DNA markers for 8q24.1.