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Related Experiment Videos

Counterflow in isotachophoresis: computer simulation and experimental studies

R R Deshmukh1, M Bier

  • 1Center for Separation Science, University of Arizona, Tucson 85721.

Electrophoresis
|March 1, 1993
PubMed
Summary

A new computer model predicts how fluid flow affects electrophoretic transport. Counterflow can selectively remove slower components, optimizing separations in isotachophoresis and capillary electrophoresis.

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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Computational Modeling

Background:

  • Electrophoretic techniques are crucial for separating charged molecules.
  • Understanding the impact of fluid flow on these separations is essential for optimization.
  • Previous models may not fully capture the dynamics of flow in electrophoretic systems.

Purpose of the Study:

  • To develop and validate a computer model for predicting the effects of co- and counter-current flows on electrophoretic transport.
  • To compare model predictions with experimental data from a large-scale recycling isotachophoresis apparatus.
  • To explore the potential of flow manipulation for optimizing electrophoretic separations.

Main Methods:

  • Development of a computational model simulating electrophoretic transport under various flow conditions.

Related Experiment Videos

  • Experimental validation using a custom-built large-scale recycling isotachophoresis (ITP) apparatus.
  • Analysis of steady-state properties and transient development of Kohlrausch-adjusted profiles with and without flow.

    • Main Results:

    • Model predictions showed no significant changes in steady-state properties or transient profiles with or without flow.
    • Application of counterflow in free-flow isotachophoresis (FF-ITP) demonstrated selective wash-out of slower sample components before steady state.
    • The magnitude of counterflow was identified as a tunable parameter for optimizing separation efficiency.

    Conclusions:

    • The developed computer model accurately predicts electrophoretic transport under flow conditions.
    • Counterflow in FF-ITP offers a method for enhancing separation selectivity by removing slower components early in the process.
    • The model's capabilities extend to simulating electroosmotic flow in capillary electrophoresis (CE) systems.