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Organization of the human CD9 gene
E Rubinstein1, P Benoit, M Billard
1INSERM U268, Hôpital Paul-Brousse, Villejuif, France.
Genomics
|April 1, 1993
Summary
The CD9 antigen, found on leukemia cells and platelets, has a gene structure detailed here. Its gene organization is similar to related cell surface proteins TAPA1 and CD63.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Background:
- The CD9 antigen is a 24-kDa molecule identified on B-lineage acute lymphoblastic leukemia cells and developing B lymphocytes.
- Platelets express significant levels of CD9 antigen and are responsive to CD9 antibodies, indicating functional relevance.
Purpose of the Study:
- To elucidate the gene structure of the CD9 antigen.
- To analyze the regulatory elements within the 5'-flanking region of the CD9 gene.
- To compare the CD9 gene organization with related cell surface protein genes.
Main Methods:
- Gene structure analysis involving exon-intron organization.
- Bioinformatic analysis of the 5'-flanking region for regulatory elements.
- Sequence identity comparison with TAPA1 and CD63 genes.
Main Results:
- The CD9 gene comprises 8 exons and spans over 20 kb.
- The 5'-flanking region lacks TATA/CAAT boxes but contains a GC-rich region with Sp1 and Krox/EGR binding sites.
- CD9 shares 45% and 25% amino acid identity with TAPA1 and CD63, respectively.
Conclusions:
- The CD9 gene structure is characterized by specific regulatory elements in its promoter region.
- CD9 belongs to a novel cell surface protein family, with gene organization similarities to TAPA1 and CD63.
- These findings provide insights into the transcriptional regulation and evolutionary relationships of the CD9 antigen.