Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphic SSR (simple-sequence-repeat) markers for chromosome 20

R Melis1, P Bradley, T Elsner

  • 1Eccles Institute for Human Genetics, University of Utah, Salt Lake City 84112.

Genomics
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Delayed cerebral ischaemia after subarachnoid haemorrhage: pathophysiology, diagnosis and neurotherapeutic opportunities.

Discover medicine·2026
Same author

Seascape connectivity: evidence, knowledge gaps and implications for temperate coastal ecosystem restoration practice and policy.

npj ocean sustainability·2025
Same author

Deciding whether to do elective neck dissection in patients with salivary gland tumors with no evidence of neck lymph node metastasis.

European annals of otorhinolaryngology, head and neck diseases·2025
Same author

A contemporary analysis of the pre- and intraoperative recognition of multigland parathyroid disease.

Langenbeck's archives of surgery·2023
Same author

Publisher Erratum: The Heart Failure Optimization Study (HF‑OPT): rationale and design.

Herzschrittmachertherapie & Elektrophysiologie·2023
Same author

The Heart Failure Optimization Study (HF-OPT): rationale and design.

Herzschrittmachertherapie & Elektrophysiologie·2023
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Researchers developed new simple-sequence repeat (SSR) markers specific to chromosome 20. Sixteen markers were genetically mapped, with ten showing high heterozygosity for potential use as index markers.

Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Development of polymorphic markers is crucial for genetic mapping and understanding chromosome structure.
  • Simple-sequence repeats (SSRs) are highly variable regions of DNA, making them valuable genetic markers.

Purpose of the Study:

  • To develop and characterize novel chromosome 20-specific simple-sequence repeat (SSR) markers.
  • To assess the polymorphic potential and genetic mapping utility of these newly developed SSRs.

Main Methods:

  • Utilized a flow-sorted phage library (LL20NS01) for chromosome 20 DNA.
  • Screened clones with a tetranucleotide repeat (AAAG)6 to identify SSR loci.
  • Sequenced 100 clones, designed primers for 39, and tested for polymorphism.
  • Mapped 16 polymorphic markers using the CEPH linkage database (version 5).

Related Experiment Videos

Main Results:

  • Successfully developed 22 polymorphic SSR markers from 39 primer sets.
  • Genetically mapped 16 of these novel markers to specific regions on chromosome 20.
  • Identified 10 SSRs with heterozygosity indices exceeding 70%, indicating their potential as index markers.

Conclusions:

  • The study successfully generated a set of polymorphic SSR markers for chromosome 20.
  • These markers are valuable for genetic studies, linkage analysis, and potential diagnostic applications.
  • Ten SSRs demonstrate high potential for use as index markers due to their significant heterozygosity.