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Related Experiment Videos

A process for enhancing selectivity and limiting plugging in plasmafractionation for APOB removal

C Legallais1, M Y Jaffrin

  • 1URA CNRS 858, Biomechanics and Medical Instrumentation, Technological University of Compiegne, France.

The International Journal of Artificial Organs
|February 1, 1993
PubMed
Summary

A novel filtration method using pulsed flow enhanced the removal of Apolipoprotein B (ApoB) while reducing losses of albumin and Apolipoprotein A1 (ApoA1). This improved the selectivity of the fractionation process.

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Area of Science:

  • Biomedical Engineering
  • Biochemical Engineering
  • Membrane Science

Background:

  • Apolipoprotein B (ApoB) is a key target for cardiovascular disease risk reduction.
  • Efficient removal of ApoB from plasma is crucial for therapeutic applications.
  • Traditional filtration methods can lead to significant loss of beneficial proteins like albumin and ApoA1.

Purpose of the Study:

  • To investigate a new filtration process utilizing pressure and flow pulsations for Apolipoprotein B (ApoB) removal.
  • To compare the performance of Eval 4A and cellulose PF100 filters under pulsed flow versus classical dead-end filtration.
  • To assess the impact of pulsed flow on the sieving coefficients and recovery of albumin and ApoA1.

Main Methods:

  • A novel filtration process employing pressure and flow pulsations was implemented in a cascade filtration setup.

Related Experiment Videos

  • Eval 4A and cellulose PF100 filters were tested under pulsed conditions and compared to dead-end filtration.
  • Plasma flux was maintained at 18 ml/(min m2) during experiments.
  • Apparent sieving coefficients for ApoB, albumin, and ApoA1 were measured.

    • Main Results:

    • Pulsed flow filtration resulted in natural retentate flow vanishing, eliminating the need for line clamping and reducing albumin and ApoA1 losses.
    • Apparent sieving coefficients for albumin and ApoA1 increased with pressure under pulsed conditions, unlike in dead-end mode.
    • ApoB sieving coefficients remained consistently low (<0.02) in both pulsed and dead-end modes.
    • Transmembrane pressure rise was slower with pulsed flow, indicating reduced membrane plugging.

    Conclusions:

    • The pulsed flow filtration process enhances selectivity by increasing the recovery of albumin and ApoA1 while maintaining efficient ApoB removal.
    • This method offers a promising alternative to conventional filtration for improved plasma fractionation.
    • Reduced membrane plugging and enhanced protein recovery contribute to a more efficient and effective therapeutic process.