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Related Experiment Videos

Terminal complement complex C5b-9 stimulates mitogenesis in 3T3 cells

J A Halperin1, A Taratuska, A Nicholson-Weller

  • 1Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.

The Journal of Clinical Investigation
|May 1, 1993
PubMed
Summary
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The membrane attack complex (MAC) of complement can trigger cell proliferation without causing cell death. This novel mitogenic effect of MAC may play a role in tissue repair and disease.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The membrane attack complex (MAC) of complement can alter cell membrane permeability, causing transient ion changes without cell lysis.
  • Ion fluxes are critical signaling events in growth factor-induced cell proliferation.

Purpose of the Study:

  • To investigate the potential mitogenic effects of MAC-induced membrane permeability changes.
  • To determine if MAC can stimulate cell proliferation independently or in synergy with other growth factors.

Main Methods:

  • Utilized purified terminal complement components to treat quiescent murine 3T3 cells.
  • Assessed cell proliferation in response to MAC, serum, and platelet-derived growth factor (PDGF).

Main Results:

Related Experiment Videos

  • Demonstrated a mitogenic effect of MAC on quiescent murine 3T3 cells.
  • Showed that MAC enhances the mitogenic effects of serum and PDGF.
  • Confirmed MAC's ability to stimulate cell proliferation even without other exogenous growth factors.

Conclusions:

  • MAC possesses a novel mitogenic activity, stimulating cell proliferation through reversible membrane permeability changes.
  • This MAC-induced mitogenesis could contribute to tissue repair processes.
  • Pathological cell proliferation at sites of complement activation may also be influenced by MAC.