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Cloning and chromosomal mapping of mouse seminal vesicle protein F

H Yoo-Warren1, A G Willse, J Hull

  • 1Institute for Metabolic Disorders, Miles Research Center, West Haven, Connecticut 06516.

The Journal of Experimental Zoology
|May 1, 1993
PubMed

Insights

Seminal vesicle proteins (SVPs) are produced by male rodents to form a copulatory plug. Unexpectedly, SVP F-related gene transcripts are highly expressed in mouse skeletal muscle, not just seminal vesicles.

Area of Science:

  • Molecular Biology
  • Genetics
  • Reproductive Biology

Background:

  • Seminal vesicle proteins (SVPs) are key components in rodent reproductive biology, forming the copulatory plug post-mating.
  • The specific functions and expression patterns of certain SVPs, like SVP F, are not fully elucidated in all species.

Purpose of the Study:

  • To investigate the nucleotide sequence and expression of a mouse clone homologous to rat SVP F.
  • To determine the chromosomal location of the mouse SVP F gene.

Main Methods:

  • Partial nucleotide sequencing of a mouse clone.
  • Gene expression analysis using transcript analysis in mouse tissues.
  • Chromosomal mapping utilizing somatic cell hybrid lines.

Main Results:

  • A partial nucleotide sequence of a mouse clone homologous to rat SVP F was obtained.
  • SVP F-related transcripts were unexpectedly found at high levels in mouse skeletal muscle.
  • The mouse SVP F gene was mapped to mouse chromosome 15.

Conclusions:

  • Mouse SVP F exhibits an unexpected expression pattern, with significant presence in skeletal muscle.
  • This finding suggests potential roles for SVP F beyond seminal vesicle function.
  • Further research is warranted to explore the function of SVP F in skeletal muscle.

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