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Growth is compromised in rats fed ozone-treated casein
T Kasai1, A Iwashita, S Kiriyama
1Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
The Journal of Nutrition
|May 1, 1993
Summary
Ozonolysis of casein modifies its amino acid profile, impacting rat growth and digestibility. While modified casein shows comparable biological value, its true digestibility is reduced, leading to heavier organs in rats.
Area of Science:
- Biochemistry
- Nutrition Science
- Food Chemistry
Background:
- Casein modification via ozonolysis aims to alter aromatic amino acid content.
- Ozonolysis can affect amino acid residues beyond phenylalanine, potentially impacting nutritional quality.
Purpose of the Study:
- To evaluate the nutritional quality of ozonated casein (OC) and pepsin-predigested ozonated casein (POC) in rat diets.
- To assess the impact of casein ozonolysis on rat growth, biological value, and true digestibility.
Main Methods:
- Casein was modified using ozonolysis, with and without prior pepsin digestion.
- Rats were fed diets containing modified casein (OC, POC), native casein (8C-AA), or an amino acid mixture (7.6AA).
- Growth, biological value, true digestibility, and organ weights were measured.
Main Results:
- Rats fed OC diets showed significantly lower growth compared to control groups.
- Biological values of OC and POC were comparable to native casein, but true digestibility of OC was lower.
- POC showed improved true digestibility over OC, but without improved growth; both OC and POC diets led to heavier kidneys and ceca.
Conclusions:
- Ozonolysis of casein alters its nutritional properties, negatively affecting rat growth.
- While pepsin predigestion improves true digestibility of ozonated casein, it does not enhance growth performance.
- Further investigation is needed to understand the organ weight changes associated with modified casein consumption.