A method for marking and relocating identified cells in whole tissue for electron microscopy

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Updated: Aug 15, 2026

Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell
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Correlative Confocal and 3D Electron Microscopy of a Specific Sensory Cell

Published on: July 19, 2015

W G Hopkins

    Journal of Microscopy
    |January 1, 1979
    PubMed

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    Immunogold Electron Microscopy

    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.