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Morphological changes in thrombocytes during blood bank storage. An ultrastructural morphometric study
1Institut für Anatomie, Medizinische Universität zu Lübeck, Deutschland.
Summary
Platelet concentrate (PC) storage causes significant ultrastructural damage, including granule reduction and open canalicular system (OCS) expansion. These findings highlight the need for improved PC preparation and storage methods to maintain platelet quality.
Area of Science:
- Hematology
- Transfusion Medicine
- Cell Biology
Background:
- Platelet concentrate (PC) storage lesion monitoring typically uses metabolic and light microscopy methods.
- Ultrastructural changes during PC storage are less frequently described.
- Key organelles like alpha-granules, lysosomal granules, and the open canalicular system (OCS) are crucial for platelet function.
Purpose of the Study:
- To investigate ultrastructural changes in platelet organelles during PC storage.
- To quantify morphometric alterations in granules and the OCS.
- To assess the impact of storage on platelet morphology and integrity.
Main Methods:
- Preparation of single-donor PCs using a CS-3000 plus processor.
- Storage of PCs under routine conditions.
- Morphometric analysis of alpha-granules, lysosomal granules, and the OCS.
Main Results:
- Significant decrease in granule volume fraction to 43% after 8 days.
- Significant increase in OCS fraction to 143% after 8 days.
- Observed morphological changes in granules, OCS development, platelet ballooning, and lysis within 3 days.
Conclusions:
- Routine PC storage leads to substantial ultrastructural degradation.
- Morphometric analysis reveals significant changes in platelet organelles.
- Current preparation and storage conditions require improvement to preserve PC quality and function.