A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development
A J Hanzlik1, M M Osemlak-Hanzlik, M A Hauser
1University of Michigan Medical School, Howard Hughes Medical Institute, Ann Arbor 48109-0650.
Abstract:
To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
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