Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

V-region directed selection in differentiating B lymphocytes

F Huetz1, L Carlsson, U C Tornberg

  • 1Unit for Applied Cell and Molecular Biology, University of Umeå, Sweden.

The EMBO Journal
|May 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Health status and health care utilization after discharge from geriatric in-hospital stay - description of a register-based study.

BMC health services research·2021
Same author

Prognostic role of serum thymidine kinase 1 kinetics during neoadjuvant chemotherapy for early breast cancer.

ESMO open·2021
Same author

Life situation 5 years after subarachnoid haemorrhage.

Acta neurologica Scandinavica·2017
Same author

Molecular subtype and tumor characteristics of breast cancer metastases as assessed by gene expression significantly influence patient post-relapse survival.

Annals of oncology : official journal of the European Society for Medical Oncology·2014
Same author

'Background' immunoglobulin production: measurement, biological significance and regulation.

Immunology today·2014
Same author

Natural antibodies and autoimmunity.

Immunology today·2014

B cell differentiation involves selection of VH gene rearrangements. In adult mice, functional VH7183 rearrangements decrease as B cells mature and move from bone marrow to spleen.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • The B cell receptor (BCR) repertoire is shaped by V(D)J recombination.
  • VH7183 gene family rearrangements are crucial for B cell development.

Purpose of the Study:

  • To analyze VH7183 rearrangements during B cell differentiation in mice.
  • To understand the selection processes acting on VH regions.

Main Methods:

  • Nucleotide sequence analysis of VH7183-D-JH rearrangements.
  • Isolation of B cells from different developmental stages (pre-B, mature B) and tissues (bone marrow, spleen).

Main Results:

  • A sequential accumulation of FL16 family D segments and depletion of certain reading frames occurred in functional rearrangements.

Related Experiment Videos

  • VH7183.1 was highly utilized (60-80%) across all B cell populations.
  • Functional VH7183 rearrangements were abundant in neonates but >96% were non-functional in adult spleen.

    • Conclusions:

    • Cellular selection acts on VH regions at multiple stages of B cell differentiation.
    • Selection pressures differ between bone marrow and peripheral B cell populations.