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Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
Published on: April 20, 2018
Hyperbaric oxygen therapy and squamous cell carcinoma cell line growth
D Sklizovic1, J R Sanger, E P Kindwall
1Department of Otolaryngology and Human Communication, Medical College of Wisconsin, Milwaukee 53226.
Head & Neck
|May 1, 1993
Summary
Hyperbaric oxygen therapy (HBO) did not significantly impact the growth of head and neck squamous cell carcinoma xenotransplants in mice. This suggests increased tissue oxygenation does not affect tumor progression in these models.
Area of Science:
- Oncology
- Biomedical Research
- Hyperbaric Medicine
Background:
- Hyperbaric oxygen (HBO) therapy enhances tissue healing through increased oxygenation.
- HBO is clinically applied post-cancer surgery or radiotherapy in head and neck regions.
- Potential for undetected viable tumors necessitates understanding HBO's effect on cancer growth.
Purpose of the Study:
- To investigate the effect of increased tissue oxygen on the growth of head and neck squamous cell carcinoma xenotransplants.
- To determine if hyperbaric oxygen influences tumor progression in a preclinical model.
Main Methods:
- Two human squamous cell carcinoma cell lines (183 and 1483) were used to create xenotransplants in mice.
- Mice received daily HBO treatment (2 atm for 90 minutes) or served as controls.
- Tumor weight, volume, and histology were assessed after 21 or 28 days of treatment.
Main Results:
- No statistically significant difference in tumor weight or volume was observed between HBO-treated and control groups.
- Histological evaluation did not reveal significant changes related to HBO exposure.
- The study found no measurable effect of increased tissue oxygen on tumor growth.
Conclusions:
- Increased tissue oxygenation via hyperbaric oxygen therapy does not significantly promote or inhibit the growth of head and neck squamous cell carcinoma xenotransplants.
- These findings suggest that HBO therapy may not directly influence the progression of this specific type of cancer.
- Further research is warranted to fully elucidate the complex interactions between HBO and various cancer types.
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