Whole-body albumin mass and distribution in rats fed on low-protein diets

1. From 5 weeks of age, control and experimental rats were given diets containing 210 and 31 g protein/kg respectively, and killed for analysis at 0, 2, 5, 8, 12 and 20 d after the start of the experiment. At these times estimates were made of plasma albumin concentration, plasma volume and total vascular and extravascular albumin mass. 2. Plasma albumin concentrations were significantly lower in the experimental animals when compared to controls at 8, 12 and 20 d but plasma volumes (ml/kg body-weight) tended to be greater in the former animals. Total vascular albumin mass (g/kg body-weight) was significantly less in experimental animals compared to controls at 8 and 20 d, but was significantly reduced below values at 0 d only at 20 d. 3. extravascular albumin mass (g/kg body-weight) was significantly lower in experimental animals in comparison with controls at 2, 5, 8, 12 and 20 d and significantly reduced below values at 0 d at 5, 8, 12, and 20 d. 4. Whole-body albumin mass was significantly reduced at 5, 8, 12 and 20 d when compared both with controls killed at the same time and animals killed at 0 d. Measurement of the ratio, extravascular albumin mass: vascular albumin mass indicated a significant redistribution of whole-body albumin mass at 5 and 20 d and mean values for this ratio were always lower in experimental animals than in controls. 5. It was concluded that measurement of plasma albumin concentration does not indicate the true extent of whole-body albumin losses in protein deficiency since total vascular albumin mass is, to some extent, maintained at the expense of extravascular albumin mass.