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Spatiotemporal expression of two cell-cell adhesion molecule 105 isoforms during liver development
N L Thompson1, M A Panzica, G Hull
1Department of Medical Oncology, Rhode Island Hospital, Brown University, Providence 02903.
Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|April 1, 1993
Summary
Cell adhesion molecule cell CAM 105 expression increases significantly at birth in rat liver, with both isoforms present in developing bile canaliculi. The long isoform appears later in development, suggesting specific roles.
Area of Science:
- Molecular Biology
- Developmental Biology
- Hepatology
Background:
- Cell CAM 105 is a rat liver adhesion molecule implicated in liver histogenesis.
- Two isoforms of cell CAM 105 exist, differing in cytoplasmic domain length and exhibiting isoform-specific phosphorylation and aggregation functions.
Purpose of the Study:
- To investigate the temporal and spatial expression of cell CAM 105 isoforms during rat liver development.
- To understand the regulatory mechanisms controlling cell CAM 105 expression during liver development.
Main Methods:
- Northern blot analysis to detect RNA expression levels.
- Immunoblot analysis to assess protein expression.
- Isoform-specific antibodies for in situ localization studies.
Main Results:
- Cell CAM 105 RNA levels are low during gestation (15-20 days) and increase significantly at birth, remaining elevated in neonatal and adult liver.
- Additional RNA species (6.0 and 2.9 kb) were detected postnatally.
- Protein expression correlated with RNA levels, indicating transcriptional regulation.
- Both isoforms were localized to maturing bile canalicular domains, with the long isoform appearing after 20 days gestation.
Conclusions:
- Cell CAM 105 expression is primarily regulated transcriptionally during rat liver development.
- The temporal expression patterns and localization of cell CAM 105 isoforms suggest distinct roles in liver histogenesis and bile canalicular development.