Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Giemsa G-banding in Allium

E B Peffley1, J N de Vries

  • 1Department of Agronotomy, Horticulture, and Entomology, Texas Tech University, Lubbock 79409.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|March 1, 1993
PubMed
Summary

G-banding, a chromosome staining technique, was successfully applied to onion (Allium cepa L.) and Welsh onion (A. fistulosum L.) chromosomes for the first time. This study visualizes G-bands in Allium species and their hybrid.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High recombination between the breakpoint of a reciprocal translocation in rye (Secale cereale L.) and an interstitially located gene.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Electrophoretic analysis of Allium alien addition lines.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Somatic embryogenesis and plant regeneration in diploid Allium fistulosum × A. cepa F1 hybrid onions.

Plant cell reports·2013
Same author

Meiotic behaviour of telo-tertiary compensating trisomics of rye: evaluation for use in hybrid varieties.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Introgression of Allium fistulosum L. into Allium cepa L.: cytogenetic evidence.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Chromosomal location of rDNA in Allium: in situ hybridization using biotin- and fluorescein-labelled probe.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Area of Science:

  • Cytogenetics
  • Plant genetics
  • Molecular biology

Background:

  • Giemsa staining is a common method for chromosome analysis.
  • G-banding is a specific Giemsa staining technique that reveals chromosome banding patterns.
  • Previous studies have not demonstrated G-banding in Allium species.

Purpose of the Study:

  • To investigate the applicability of Giemsa staining protocols for G-banding in Allium chromosomes.
  • To visualize and analyze G-banding patterns in Allium cepa L., Allium fistulosum L., and their interspecific hybrid.

Main Methods:

  • Utilized divergent Giemsa staining protocols originally developed for other plant species.
  • Applied these protocols to the chromosomes of Allium cepa L., A. fistulosum L., and their interspecific hybrid.

Main Results:

  • Successfully visualized G-bands in the chromosomes of Allium cepa L., A. fistulosum L., and their interspecific hybrid.
  • This marks the first successful demonstration of G-banding in Allium chromosomes.
  • Observed and discussed similarities and differences in G-banding patterns compared to other known patterns in Allium.

Conclusions:

  • Established G-banding as a viable technique for analyzing the chromosomes of Allium species.
  • The findings provide a new tool for cytogenetic studies in Allium.
  • Further research can explore comparative genomics and evolutionary relationships within the Allium genus using these banding patterns.

Related Experiment Videos