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08:13
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
Published on: December 27, 2016
Lymphocyte surface membrane changes in dividing cells and following regidification with mitogens
Immunology
|March 1, 1977
Summary
Lymphocyte membrane protein dynamics were studied using iodination. Protein turnover slows significantly in
Area of Science:
- Immunology and Cell Biology
- Biochemistry and Biophysics
Background:
- Surface membrane proteins are crucial for lymphocyte function.
- Understanding lymphocyte membrane dynamics is key to immunology.
Purpose of the Study:
- To investigate lymphocyte surface membrane protein dynamics.
- To compare membrane turnover rates in different lymphocyte states.
Main Methods:
- Iodination of surface membrane proteins.
- Analysis of membrane turnover rates in various lymphocyte conditions.
Main Results:
- Membrane protein turnover is reduced when receptor redistribution is inhibited by mitogens, causing membrane rigidification.
- Protein loss is slower in lymphocytes stimulated with mitogens and undergoing DNA synthesis.
Conclusions:
- Altered membrane dynamics may correlate with immunological inactivity.
- These findings could explain pathological unresponsiveness.
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