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Related Experiment Videos

Increased NGF mRNA expression in denervated rat skin

K M Mearow1, Y Kril, J Diamond

  • 1Division of Anatomy, McMaster University Medical Centre, Hamilton, Ontario, Canada.

Neuroreport
|April 1, 1993
PubMed
Summary

Following skin denervation in rats, Nerve Growth Factor (NGF) mRNA expression significantly increased in skin cells. This heightened NGF availability promotes collateral sprouting in nearby sensory nerves.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Dermatology

Background:

  • Nerve Growth Factor (NGF) is crucial for neuronal survival and function.
  • The role of NGF in skin and its regulation following nerve injury is not fully understood.

Purpose of the Study:

  • To quantify Nerve Growth Factor (NGF) mRNA expression in denervated adult rat skin.
  • To investigate the cellular localization of NGF mRNA in the skin.
  • To explore the relationship between NGF availability and collateral sprouting of sensory axons.

Main Methods:

  • Coupled reverse transcription and competitive polymerase chain reaction (RT-PCR) were used to measure NGF mRNA levels.
  • In situ hybridization was employed to determine the cellular distribution of NGF mRNA.
  • Skin denervation was performed on adult rats to create experimental conditions.

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Main Results:

  • NGF mRNA levels in denervated skin increased 5-10 fold within 2-4 days and remained elevated for at least 2 weeks.
  • NGF mRNA was detected in distal nerve pathways, dermal fibroblasts, and the basal epidermal layer.
  • Denervated skin induced NGF-dependent collateral sprouting of adjacent intact sensory axons.

Conclusions:

  • Increased NGF production and availability in denervated skin are linked to heightened NGF mRNA expression.
  • Cutaneous nerves play a regulatory role in controlling NGF production within the skin.
  • NGF in the skin supports collateral sprouting of sensory axons, indicating a role in nerve repair and plasticity.