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Related Experiment Videos

Deoxygenation-induced changes in sickle cell-sickle cell adhesion

C L Morris1, D L Rucknagel, C H Joiner

  • 1University of Cincinnati College of Medicine, Department of Pediatrics, OH.

Blood
|June 1, 1993
PubMed
Summary

Sickle cells adhere more strongly when deoxygenated, even without forming typical clumps. This increased cell sticking is linked to hemoglobin polymerization and may involve fibrinogen, suggesting new therapeutic targets for sickle cell disease.

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Area of Science:

  • Hematology
  • Biophysics
  • Cell Biology

Background:

  • Sickle cell adherence is a key factor in disease pathology.
  • Understanding cell-cell interactions is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the relationship between deoxygenation and sickle cell adhesion.
  • To identify the mechanisms underlying deoxygenation-induced cell sticking.

Main Methods:

  • Measurement of yield stress in deoxygenated sickle cell suspensions.
  • Assessment of hemoglobin polymerization via sickling and viscosity changes.
  • Evaluation of cell adhesion in buffer with and without fibrinogen.

Main Results:

  • Deoxygenation significantly increased sickle cell yield stress, indicating enhanced adhesion.

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  • Increased adhesion occurred despite reduced deformability and rouleaux formation.
  • Fibrinogen restored deoxygenation-induced adhesion in buffer, suggesting a role for plasma proteins.

    • Conclusions:

    • Deoxygenation-induced sickle cell adhesion is mediated by strong, localized membrane interactions, possibly involving spicule formation.
    • Fibrinogen and other plasma proteins may play a role in this adhesion process.
    • These findings offer insights into sickle cell pathophysiology and potential therapeutic strategies.