Related Experiment Video
Updated: Jul 24, 2026
08:59
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
Published on: October 5, 2016
Plasmanate as a medium supplement for in vitro fertilization
A Adler1, A M Reing, J M Bedford
1Department of Obstetrics and Gynecology, New York Hospital-Cornell Medical Center, New York 10021.
Journal of Assisted Reproduction and Genetics
|January 1, 1993
Summary
Plasmanate, a protein supplement, shows potential as an alternative to maternal serum in in vitro fertilization (IVF) medium. While retrospective data favored Plasmanate, prospective trials indicated otherwise, necessitating further research.
Area of Science:
- Reproductive Science
- Biotechnology
Background:
- Autologous maternal serum and Plasmanate are protein supplements used in in vitro fertilization (IVF) media.
- Plasmanate, a preparation of human serum albumin and mixed globulins, is often employed when maternal serum is unavailable or in cases of unexplained infertility, sperm antibodies, or endometriosis.
Purpose of the Study:
- To evaluate Plasmanate as a substitute for autologous maternal serum in IVF medium.
- To compare the efficacy of Plasmanate versus maternal serum in achieving clinical pregnancies.
Main Methods:
- Retrospective analysis of 1019 IVF cycles comparing Plasmanate and maternal serum use.
- Matched patient cycle comparison (450 cycles) to control for laboratory variables.
- Prospective randomized trial in first-time IVF patients with tubal infertility.
Main Results:
- Retrospective analysis showed a higher clinical pregnancy rate (34%) with Plasmanate compared to maternal serum (24%).
- Prospective randomized trial results reversed this trend, indicating a different outcome under controlled conditions.
- The study involved a significant number of IVF cycles, providing a substantial dataset for comparison.
Conclusions:
- Plasmanate may be a suitable protein substitute for maternal serum in specific IVF scenarios, particularly when serum is unavailable or unsuitable.
- Further investigation is required to definitively establish Plasmanate's role and efficacy in IVF protocols.
- The study highlights the complexity of optimizing IVF media and the need for rigorous comparative trials.
Related Concept Videos
01:21Cell Culture
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
01:24In Vitro Fertilization
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...