Related Experiment Video
Updated: Aug 8, 2026
07:48
Preparing a 68Ga-labeled Arginine Glycine Aspartate (RGD)-peptide for Angiogenesis
Published on: January 7, 2019
A specific and sensitive radioimmunoassay for rat C-peptide
J O Akpan1, L G Weide, R L Gingerich
1Department of Pediatrics, Washington University, St. Louis, MO 63110.
Summary
A new radioimmunoassay for rat C-peptide (RCP) has been developed. This sensitive and specific assay is a valuable tool for diabetes research in rodent models.
Area of Science:
- Biochemistry
- Endocrinology
- Assay Development
Background:
- Rat C-peptide (RCP) is a biomarker for insulin secretion.
- Accurate measurement of RCP is crucial for diabetes research in animal models.
Purpose of the Study:
- To develop and validate a sensitive and specific radioimmunoassay for rat serum C-peptide.
- To provide a reliable tool for studying diabetes in rodent models.
Main Methods:
- Development of a radioimmunoassay using a guinea pig anti-rat C-peptide antibody.
- Validation of the assay's sensitivity, specificity, precision, and accuracy.
- Chromatographic analysis (Sephadex G-50) to confirm RCP identity.
Main Results:
- The assay demonstrated high sensitivity (24.2 pM) and specificity with negligible cross-reactivity.
- Low intra-assay (3.5-6.1%) and between-assay (4.1-9.5%) coefficients of variation were observed.
- Mean recovery of RCP was 100.8 +/- 2%, and fasting/postprandial levels in normal rats were established.
Conclusions:
- A sensitive, specific, and reliable radioimmunoassay for rat serum C-peptide has been successfully developed and validated.
- This assay is suitable for accurate quantification of RCP in rodent serum.
- The developed assay serves as a valuable tool for diabetes research in animal models.
