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Related Experiment Videos

Monocyte urokinase expression: modulation by interleukins

M R Gyetko1, C C Wilkinson, R G Sitrin

  • 1Pulmonary Section, Department of Veterans Affairs Medical Center, Ann Arbor, MI.

Journal of Leukocyte Biology
|May 1, 1993
PubMed
Summary
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Interleukin-1 (IL-1) and Interleukin-2 (IL-2) increase both urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-2 (PAI-2) in monocytes. This results in a net increase in plasminogen activator inhibitor activity, regulating monocyte proteolytic activity.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Mononuclear phagocytes regulate proteolytic activity through urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-2 (PAI-2).
  • Understanding the regulation of these components by cytokines is crucial for comprehending monocyte function.

Purpose of the Study:

  • To investigate the regulatory effects of interleukin-1 (IL-1) and interleukin-2 (IL-2) on monocyte plasminogen activator (PA) activity.
  • To compare the expression of uPA and PAI-2 in monocytes stimulated with IL-1 and IL-2.

Main Methods:

  • Human monocyte-conditioned medium was analyzed for secreted PA and PA inhibitor activity after stimulation with recombinant IL-1 or IL-2.
  • Northern blot analysis was employed to measure steady-state mRNA levels for uPA and PAI-2.

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Main Results:

  • Both IL-1 and IL-2 significantly increased PA inhibitor activity in a dose-dependent manner (up to 11-fold).
  • IL-1 and IL-2 increased steady-state mRNA for both PAI-2 and uPA.
  • IL-1 showed a greater increase in uPA mRNA compared to IL-2.

Conclusions:

  • IL-1 and IL-2 modulate monocyte proteolytic activity by up-regulating both uPA and PAI-2 expression, with a predominant increase in PAI-2.
  • Cytokine-specific regulation of plasminogen activity is achieved through differential proportionate expression of uPA and PAI-2.