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Mesangial cell proliferation is directly enhanced by sera from patients with renal insufficiency
J Mattana1, M Abramovici, P C Singhal
1Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
We undertook the present study to determine whether there is a direct effect of sera from patients with renal insufficiency on mesangial cell proliferation. Growth-arrested mesangial cells were incubated with sera from patients with varying degrees of renal insufficiency and then pulsed with [3H]thymidine. Thymidine uptake was progressively greater with more advanced renal failure and was linearly correlated with (creatinine)-1. This data suggests that serum from patients with loss of renal function contains mitogens capable of directly enhancing mesangial cell proliferation.
We undertook the present study to determine whether there is a direct effect of sera from patients with renal insufficiency on mesangial cell proliferation. Growth-arrested mesangial cells were incubated with sera from patients with varying degrees of renal insufficiency and then pulsed with [3H]thymidine. Thymidine uptake was progressively greater with more advanced renal failure and was linearly correlated with (creatinine)-1. This data suggests that serum from patients with loss of renal function contains mitogens capable of directly enhancing mesangial cell proliferation.