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A continuous, simple and rapid method for the detection, extraction and identification of residual antibacterial
1Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
Abstract:
Most antibacterial agents produced larger inhibition zones and showed lower detectable concentrations on minimal medium (MM) seeded with Bacillus subtilis than on Mueller-Hinton medium. After simple extraction, using a small amount of acetonitrile, from an agar block inside the inhibitory zone produced by each antibacterial agent, identification was carried out by reverse-phase high-performance liquid chromatography (HPLC). It is recommended that for inspection of residues MM is superior as a bioassay medium. The continuous, simple and rapid method described may be useful for routine laboratory testing of residual antimicrobial agents in food.