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Lipid peroxidation abnormalities in hemodialyzed patients
Nephron
|January 1, 1993
Summary
Hemodialysis patients show increased oxidative damage, particularly within red blood cells (RBCs). This may stem from reduced antioxidant enzyme activities, like superoxide dismutase (SOD) and glutathione peroxidase (GPX).
Area of Science:
- Biochemistry
- Nephrology
- Oxidative Stress Research
Background:
- Hemodialysis is a life-sustaining treatment for kidney failure.
- Potential for oxidative damage during hemodialysis requires investigation.
Purpose of the Study:
- To assess oxidative damage in patients undergoing regular dialysis treatment (RDT).
- To evaluate the role of antioxidant systems in hemodialysis-associated oxidative stress.
Main Methods:
- Measured plasma conjugated dienes (CD) and thiobarbituric acid (TBA) reactants in plasma and red blood cells (RBCs).
- Assessed extracellular antioxidant systems: plasma antioxidant activity, tocopherol, urate, transferrin, haptoglobin, and ceruloplasmin.
- Determined RBC superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities.
Main Results:
- RBC TBA reactant concentration was significantly elevated in RDT patients compared to healthy subjects.
- RBC SOD and GPX activities were significantly lower in RDT patients.
- An inverse correlation was observed between decreased RBC GPX and increased RBC TBA reactant concentration.
Conclusions:
- Patients on regular dialysis treatment experience significant intracellular oxidative stress.
- Reduced RBC superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities contribute to this oxidative stress.
- Oxidative damage is a notable concern during hemodialysis.