Detection of c-mos proto-oncogene expression in human cells

Related Experiment Video

Updated: Nov 6, 2026

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
11:30

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

Published on: December 18, 2010

C C Li1, E Chen, C D O'Connell

  • 1Biological Carcinogenesis and Development Program, Inc./DynCorp, Frederick, Maryland 21702.

Oncogene
|June 1, 1993
PubMed

Insights

The c-mos proto-oncogene protein is expressed in human somatic cells, including neuroblastoma and cervical carcinoma cell lines. This ubiquitous expression suggests a fundamental role for c-mos in cellular functions.

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • The human c-mos proto-oncogene has been studied for over a decade.
  • Little is known about its protein product and expression in somatic cells.

Purpose of the Study:

  • To detect and characterize c-mos protein expression in human somatic cells.
  • To investigate the role of the c-mos proto-oncogene.

Main Methods:

  • Generated c-mos-specific antisera.
  • Utilized Western blot, immunoprecipitation, Northern blot, and RT-PCR analyses.
  • Examined human neuroblastoma and cervical carcinoma cell lines.

Main Results:

  • Detected c-mos protein (p37 major, p40/p35 minor forms) in SK-N-BE2 cells.
  • Identified 3.5- and 1.7-kb c-mos RNA messages.
  • Confirmed ubiquitous c-mos RNA detection across all examined human samples.
  • Showed c-mos protein expression in cervical carcinoma cell lines.

Conclusions:

  • c-mos protein is expressed in human somatic cells.
  • Ubiquitous low-level expression suggests a fundamental role for the c-mos proto-oncogene.

More Related Videos

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo05:44

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Published on: April 25, 2016

Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line06:24

Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line

Published on: April 11, 2025

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...