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Bacteriophage PRD1 proteins: cross-linking and scanning transmission electron microscopy analysis
C Luo1, J Hantula, W Tichelaar
1Department of Genetics, University of Helsinki, Finland.
Virology
|June 1, 1993
Abstract:
Bacteriophage PRD1, a double-stranded DNA virus infecting Escherichia coli, has a membrane inside the protein capsid. Chemical cross-linking and scanning transmission electron microscopy showed that the multimeric major coat protein (P3) exists in a trimeric form. Cross-linking revealed, in addition, that protein P11, located between the protein coat and the membrane, exists also as a homotrimer. Minor protein P7 was associated with the major coat protein P3. Under nonreducing conditions the infectivity proteins P16 and P18 formed homomultimeric complexes which were dissociated upon addition of 2-mercaptoethanol.