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Related Experiment Videos

Barrier function regulates epidermal lipid and DNA synthesis

E Proksch1, W M Holleran, G K Menon

  • 1Dermatology Service, Veterans Administration Medical Center, San Francisco, CA.

The British Journal of Dermatology
|May 1, 1993
PubMed
Summary

The skin

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Ultrastructure of skin from Refsum disease with emphasis on epidermal lamellar bodies and stratum corneum barrier lipid organization.

Archives of dermatological research·2014

Area of Science:

  • Dermatology and Skin Biology
  • Epidermal Barrier Function
  • Cellular Signaling in Skin

Background:

  • The stratum corneum acts as a crucial permeability barrier.
  • Its structure relies on corneocytes and an intercellular lipid matrix.
  • Lipid synthesis and delivery are key to epidermal differentiation and barrier maintenance.

Purpose of the Study:

  • To investigate the relationship between epidermal barrier disruption and the synthesis of lipids and DNA.
  • To elucidate the mechanisms of skin barrier repair.
  • To explore the role of specific enzymes and fatty acids in barrier homeostasis.

Main Methods:

  • Topical acetone treatment to disrupt the skin barrier.
  • Measurement of lipid and DNA synthesis rates.
  • Inhibition of key enzymes (HMG CoA reductase, SPT) and topical application of fatty acids.
  • Occlusion studies to simulate barrier repair.

Main Results:

  • Barrier disruption stimulates synthesis of free fatty acids, sphingolipids, and cholesterol.
  • Key enzymes HMG CoA reductase and SPT activity increase post-disruption.
  • Inhibiting these enzymes or applying essential fatty acids impacts barrier repair.
  • Both lipid and DNA synthesis increase following barrier damage, suggesting a dual repair mechanism.

Conclusions:

  • Cholesterol and sphingolipids are vital for epidermal barrier function and repair.
  • Stimulated DNA synthesis leading to epidermal hyperplasia is a secondary repair mechanism.
  • Understanding these pathways is relevant to hyperproliferative skin diseases.

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