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The cDNA sequence of horse transferrin
1Gene Mapping Unit, AgResearch Grasslands Research Centre, Palmerston North, New Zealand.
Biochimica Et Biophysica Acta
|May 28, 1993
Summary
Researchers sequenced horse transferrin cDNA, revealing a 2305 bp sequence with 80% identity to human transferrin. This provides insights into equine iron transport and protein structure.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Transferrin is a crucial iron-binding protein involved in iron transport in vertebrates.
- Understanding transferrin sequences across species aids in comparative genomics and evolutionary studies.
Purpose of the Study:
- To determine the complete cDNA sequence of horse transferrin.
- To analyze the structural and functional characteristics of horse transferrin.
Main Methods:
- Isolation of cDNA clones from a horse liver cDNA library.
- Polymerase Chain Reaction (PCR) amplification of specific gene regions.
- DNA sequencing of isolated and amplified clones.
Main Results:
- The full-length horse transferrin cDNA sequence was determined to be 2305 bp.
- The sequence exhibited 80% identity to human transferrin cDNA.
- The encoded protein consists of 706 amino acids, including a 19-amino acid signal sequence.
- Conserved features characteristic of the transferrin family, including duplicated structure and iron-binding residues, were identified.
Conclusions:
- The determined horse transferrin cDNA sequence provides a valuable resource for further research.
- The findings confirm the conserved nature of transferrin structure and function across mammalian species.
- This study contributes to the understanding of iron metabolism and transport in horses.