Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite...

We have targeted the mini-exon of Leishmania amazonensis, the sequence present at the 5' end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.