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Preparation and in vitro evaluation of "no-carrier-added" 18F-labeled biotin
1Brain Imaging Center, University of California, Irvine 92717.
Nuclear Medicine and Biology
|May 1, 1993
Abstract:
This paper will describe the preparation of "no-carrier-added" 18F-labeled biotin where the radiolabel bound to an aromatic moiety is described. This has been accomplished by preparation of [18F]fluorobenzylbromide (yield 20-30%) and its reaction with biotin-LC-hydrazide. This yielded "no-carrier-added" radiolabeled biotin (5-10%) which was then purified by reversed phase HPLC. The pure product was found to bind to avidin, thereby demonstrating retention of its biological integrity. Thus this product is potentially useful for imaging tumor tissue following injection of avidin coupled MoAbs.