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Related Experiment Videos

Glutamine stability in biological tissues evaluated by fluorometric analysis

V B Grossie1, J Yick, M Alpeter

  • 1Department of General Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.

Clinical Chemistry
|June 1, 1993
PubMed
Summary

Glutamine is stable in plasma and tissue extracts at low pH, enabling accurate quantification. This amino acid can be reliably measured using fluorometric analysis after storage at -70°C.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Glutamine has been historically considered unstable, posing challenges for accurate quantification.
  • Recent findings suggest glutamine stability at low pH ranges warrants further investigation.

Purpose of the Study:

  • To evaluate the stability and quantitation of glutamine in plasma and tissue extracts.
  • To assess the efficacy of fluorometric analysis for glutamine measurement.

Main Methods:

  • Fluorometric analysis was employed to measure glutamine concentrations.
  • Stability was assessed in aqueous solutions, trichloroacetic acid (TCA) solutions, plasma, and tissue extracts under various storage conditions (-70°C).
  • Linearity and coefficient of variation were determined for aqueous glutamine solutions.

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Main Results:

  • Glutamine concentration in aqueous solutions showed linear variation up to 0.8 mmol/L (r2 = 98.7).
  • Glutamine in aqueous solution was stable for at least 16 days at -70°C.
  • Plasma glutamine concentrations were stable for over a year when stored at -70°C.
  • Glutamine extraction from rat sarcoma and liver tissue using 50 g/L TCA was highly efficient (88.6% and 90.2%, respectively).
  • Extracted glutamine remained stable in TCA for at least 7 days at -70°C without neutralization.

Conclusions:

  • Glutamine is stable in plasma and tissue extracts, particularly at low pH.
  • Fluorometric analysis is a convenient and accurate method for quantifying glutamine using small sample volumes.
  • The findings support reliable glutamine quantitation in biological samples for research and clinical applications.