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Related Experiment Videos

Functional coupling between sarcoplasmic-reticulum-bound creatine kinase and Ca(2+)-ATPase

P Korge1, S K Byrd, K B Campbell

  • 1Dept. VCAPP, Washington State University, Pullman 99164.

European Journal of Biochemistry
|May 1, 1993
PubMed
Summary

Membrane-bound creatine kinase in fast skeletal muscle regenerates ATP locally for the calcium pump. This functional coupling supports efficient calcium (Ca2+) uptake by sarcoplasmic reticulum vesicles.

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Area of Science:

  • Biochemistry
  • Muscle Physiology

Background:

  • Sarcoplasmic reticulum (SR) membranes in fast skeletal muscle contain creatine kinase (CK).
  • CK plays a role in energy buffering and ATP regeneration.

Purpose of the Study:

  • To investigate the role of membrane-bound CK in local ATP regeneration.
  • To determine the physiological significance of this regeneration for calcium pump function.

Main Methods:

  • Investigated ATP regeneration by membrane-bound CK using sarcoplasmic reticulum (SR) vesicles.
  • Assessed the effect of ADP accumulation on Ca(2+)-uptake by SR vesicles.
  • Examined the influence of localized ATP regeneration on Ca(2+)-ATPase activity.

Main Results:

  • ADP produced by Ca(2+)-ATPase is efficiently phosphorylated by CK using phosphocreatine.

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  • CK-mediated ATP regeneration supports Ca(2+)-uptake by preventing ADP accumulation.
  • Regenerated ATP is preferentially used by Ca(2+)-ATPase, indicating functional coupling with CK.

    • Conclusions:

    • Functional coupling exists between CK and Ca(2+)-ATPase on SR membranes.
    • This coupling is crucial for maintaining efficient calcium pump function in skeletal muscle.
    • Factors like CK amount and phosphocreatine levels influence this coupling and Ca(2+)-pump activity.