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Adrenal kallikrein

H Nolly1, G Saed, O A Carretero

  • 1Henry Ford Hospital, Hypertension and Vascular Research Division, Detroit, MI 48202.

Hypertension (Dallas, Tex. : 1979)
|June 1, 1993
PubMed
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Glandular kallikrein was found in rat adrenal glands, existing in active and inactive forms. Messenger RNA presence indicates local synthesis, suggesting a role in adrenal function regulation.

Area of Science:

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Background:

  • Kallikreins are serine proteases involved in various physiological processes.
  • The adrenal gland's role in regulating bodily functions is well-established.
  • Local kallikrein-kinin systems are implicated in tissue-specific functions.

Purpose of the Study:

  • To identify and characterize kallikrein in rat adrenal glands.
  • To investigate the presence of glandular kallikrein mRNA in the adrenal tissue.
  • To explore the potential local synthesis and function of kallikrein within the adrenal gland.

Main Methods:

  • Enzyme activity assays to measure kininogenase activity.
  • Inhibition studies using specific kallikrein inhibitors and antibodies.

Related Experiment Videos

  • Gel filtration chromatography and alkaline polyacrylamide gel electrophoresis for enzyme characterization.
  • Polymerase chain reaction (PCR) and Southern blotting to detect glandular kallikrein mRNA.

    • Main Results:

    • Kininogenase activity was detected in rat adrenal glands, increasing after trypsin preincubation.
    • Adrenal kininogenase activity was significantly inhibited by phenylmethylsulfonyl fluoride, D-Phe-Phe-Arg-chloromethyl ketone, and aprotinin.
    • Antibodies against rat urinary kallikrein strongly inhibited adrenal kininogenase activity.
    • Immunoreactive glandular kallikrein was quantified, and its molecular weight was estimated at 33,000 D.
    • Glandular kallikrein mRNA was detected in adrenal gland RNA via PCR and Southern blotting.
    • Adrenal kallikrein exhibited similar electrophoretic mobility to purified submandibular gland kallikrein.

    Conclusions:

    • Glandular kallikrein is present in rat adrenal glands in both active and inactive forms.
    • The detection of glandular kallikrein mRNA suggests local synthesis within the adrenal gland.
    • This finding supports the potential involvement of a local kallikrein-kinin pathway in regulating adrenal function.