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Analysis of human sera for filarial IgM antibody using antigen fractions isolated from immune complexes
1School of Life Sciences, Ravishankar University, Raipur, M.P., India.
Filarial IgM antibody response to various antigenic fractions isolated from immune complexes by SDS-PAGE viz., IC-2, IC-4, IC-7, and IC-9, was studied by ELISA. The IgM antibody response to almost all antigenic fractions was seen more in microfilaraemic group compared to the clinical filariasis group. The antibody response to IC-9 antigen fraction as compared to other fractions was found significantly higher in microfilaraemic patients than in patients with clinical manifestations. Ninety percent of microfilariae carriers and 75% of clinical filarial patients have shown IgM isotype to IC-9 antigen. Studies with various lectins have revealed the Con A binding nature of IC-9 antigen. This study suggests that IC-9 antigen could be another candidate antigen in the immunodiagnosis of lymphatic filariasis.
Filarial IgM antibody response to various antigenic fractions isolated from immune complexes by SDS-PAGE viz., IC-2, IC-4, IC-7, and IC-9, was studied by ELISA. The IgM antibody response to almost all antigenic fractions was seen more in microfilaraemic group compared to the clinical filariasis group. The antibody response to IC-9 antigen fraction as compared to other fractions was found significantly higher in microfilaraemic patients than in patients with clinical manifestations. Ninety percent of microfilariae carriers and 75% of clinical filarial patients have shown IgM isotype to IC-9 antigen. Studies with various lectins have revealed the Con A binding nature of IC-9 antigen. This study suggests that IC-9 antigen could be another candidate antigen in the immunodiagnosis of lymphatic filariasis.