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Heteroduplex formation: a potential source of genotyping error from PCR products
S L Hatcher1, Q T Lambert, R L Teplitz
1University of California, Davis Medical Center, Department of Pathology, Sacramento 95816.
Prenatal Diagnosis
|March 1, 1993
Abstract:
The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease-causing alleles. We present evidence illustrating that heterozygous PCR products can manifest 'invisible' heteroduplexes that can ultimately lead to genotyping errors. Justifications for these 'invisible' heteroduplexes and requisite parameters to optimize their detection are presented.