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Visualization of ultraviolet light-induced thymine dimers in DNA by immunoelectron microscopy
M U Udono1, M Hori, M Hirayama
1Department of Dermatology, Nagasaki University School of Medicine, Japan.
Photochemistry and Photobiology
|April 1, 1993
Abstract:
To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.