Detection of mucosal immune responses in chickens after immunization or infection

In order to measure mucosal antibody responses in the chicken intestine an ELISA-based assay was developed that was able to detect antigen-specific antibodies in an isotype-specific way. Locally produced antibodies could be detected after overnight culture at 37 degrees C. In particular the production of IgA, more than IgM and IgG, was significantly increased by immunization of the animals with K99 pilus antigen or by infection with Eimeria tenella parasites. Data presented here indicate that the assay can be used to estimate the magnitude of the mucosal antibody response in experimental conditions where antibody levels in bile or intestinal contents were not significantly changed.