Quantitative in vivo assessment of the tissue response to dermal sheep collagen in abdominal wall defects

We quantified the tissue response, tissue organization and patency of biodegradable patches for the repair of abdominal wall defects. We used dermal sheep collagen, cross-linked with hexamethylenediisocyanate in a model. The collagen patches were implanted either untreated or plasma polymerized with tetrafluoroethylene (TFE), to improve the properties of the patch for bowel adhesion and rate of degradation. The implants with surrounding tissue were retrieved after 3 d, 1, 2, 4, 6 and 8 wk, then sectioned and stained specifically for macrophages, neutrophils, T-lymphocytes and endothelium. Only macrophages and neutrophils were observed in the implant and surrounding tissue, with different antigen expression in the macrophages. This was found to be dependent on whether the macrophages were found within the implant or in the surrounding tissue. The neutrophils and macrophages were assessed using image analysis techniques to quantify the tissue responses to treated and untreated collagen, enabling comparison of the respective tissue responses. No significant differences were found between the two forms of this collagen. Infection played a key role in the severity of the tissue response around both types of implants, resulting in large variations in cell counts at each time period. Treating the collagen with TFE did not significantly improve its performance in this application.