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Related Experiment Videos

Alterations in circulating blood volume during polymicrobial sepsis

P Wang1, Z F Ba, S M Tait

  • 1Department of Surgery, Michigan State University, East Lansing 48824-1315.

Circulatory Shock
|June 1, 1993
PubMed
Summary

Circulating blood volume (CBV) remains stable in early sepsis but decreases in late sepsis. Liver injury markers and hepatic microcirculation increase during sepsis, but CBV assessment may be limited.

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Area of Science:

  • Sepsis Pathophysiology
  • Cardiovascular Physiology
  • Hepatology

Background:

  • Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection.
  • While sepsis pathophysiology is extensively studied, the impact on circulating blood volume (CBV) remains unclear.
  • Understanding CBV changes is crucial for managing septic shock and improving patient outcomes.

Purpose of the Study:

  • To investigate alterations in circulating blood volume (CBV) during the progression of sepsis.
  • To assess liver injury and hepatic microcirculation in a rat model of sepsis.
  • To evaluate the utility of in vivo indocyanine green (ICG) clearance for CBV assessment in sepsis.

Main Methods:

  • Sepsis was induced in rats using cecal ligation and puncture (CLP).

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  • Circulating blood volume (CBV) was measured using in vivo indocyanine green (ICG) clearance at various time points.
  • Liver enzymes (SGPT, SGOT) and hepatic microcirculation were assessed to evaluate organ damage and blood flow.

    • Main Results:

    • CBV remained unchanged in early sepsis (up to 10 hours post-CLP) but significantly decreased in late sepsis (20 hours post-CLP).
    • Systemic hematocrit increased, indicating plasma volume reduction, while liver enzymes (SGPT, SGOT) and hepatic microcirculation significantly increased.
    • ICG clearance method may have limitations in accurately assessing CBV during the hyperdynamic phase of sepsis (5-10 hours post-CLP).

    Conclusions:

    • Circulating blood volume is preserved in early sepsis but declines in later stages.
    • Sepsis is associated with significant liver injury and enhanced hepatic microcirculation.
    • The indocyanine green clearance technique may require careful interpretation for CBV assessment in hyperdynamic sepsis.