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Cannabinoid-receptor expression in human leukocytes

M Bouaboula, M Rinaldi, P Carayon

    European Journal of Biochemistry
    |May 15, 1993
    PubMed
    Summary
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    Cannabinoid receptors are present in human immune tissues, including spleen, tonsils, and leukocytes. This discovery provides a molecular basis for how cannabinoids affect the immune system.

    Area of Science:

    • Immunology
    • Neuroscience
    • Molecular Biology

    Background:

    • Cannabinoids, derived from marijuana, significantly impact the central nervous system (CNS) via cannabinoid receptors.
    • While cannabinoid effects on the immune system are known, receptor presence was previously limited to the brain and testis.
    • The molecular mechanisms underlying cannabinoid-immune interactions remained largely uncharacterized at the periphery.

    Purpose of the Study:

    • To investigate the expression of cannabinoid receptors in human immune tissues.
    • To quantify cannabinoid receptor mRNA levels in various human blood cell subpopulations.
    • To confirm cannabinoid receptor presence at both mRNA and protein levels on human leukocytes.

    Main Methods:

    • Utilized a sensitive polymerase-chain-reaction (PCR)-based method for mRNA quantification.

    Related Experiment Videos

  • Analyzed cannabinoid receptor mRNA expression in human spleen, tonsils, and peripheral blood leukocytes.
  • Determined receptor binding kinetics using a synthetic cannabinoid ligand ([3H]CP-55940) on U937 cell membranes.

    • Main Results:

    • Cannabinoid receptor transcripts were detected in human spleen, tonsils, and peripheral blood leukocytes, albeit at lower levels than in the brain.
    • Significant variations in cannabinoid receptor mRNA levels were observed across different blood cell types, with B cells showing the highest expression.
    • Cannabinoid receptor expression was confirmed at the protein level on U937 cell membranes, with a Kd of 0.1 nM and Bmax of 525 fmol/mg protein.

    Conclusions:

    • The study demonstrates the presence of cannabinoid receptors at both mRNA and protein levels in human immune cells.
    • This finding establishes a molecular foundation for understanding cannabinoid interactions with the human immune system.
    • The widespread expression in immune tissues suggests a significant role for cannabinoid signaling in immune function.