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Immunoassay reagents for psychoactive drugs. I. The method for the development of antibodies specific to
M Adamczyk1, J Fishpaugh, C Harrington
1Abbott Laboratories, Abbott Park, IL 60064.
Journal of Immunological Methods
|June 4, 1993
Abstract:
Amitriptyline and nortriptyline were structurally modified by the attachment of spacer arms to the aromatic ring which were subsequently attached to bovine serum albumin (BSA). Rabbits inoculated with these conjugates yielded polyclonal antisera with high selectivity and good titers. This approach required novel spacer arms and new conjugation methods. The antisera produced were characterized with respect to their cross-reactivity with amitriptyline, nortriptyline and their hydroxy metabolites as well as selected structurally related compounds.