Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Distinct NCAM splicing events are differentially regulated during rat brain development

A A Reyes1, S V Schulte, S Small

  • 1Division of Basic Research, Children's Hospital Research Foundation, Cincinnati, OH 45229.

Brain Research. Molecular Brain Research
|March 1, 1993
PubMed
Summary

Neural cell adhesion molecule (NCAM) microsplicing patterns change during brain development. The VASE exon increases in all NCAM forms, while other exons are specific to smaller NCAM isoforms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of disorder-free localization using a (2+1)D lattice gauge theory on a quantum processor.

Science (New York, N.Y.)·2026
Same author

Visualizing dynamics of charges and strings in (2 + 1)D lattice gauge theories.

Nature·2025
Same author

Scaling and logic in the colour code on a superconducting quantum processor.

Nature·2025
Same author

Thermalization and criticality on an analogue-digital quantum simulator.

Nature·2025
Same author

Corrigendum to 'Efficacy of a standard meticillin-resistant Staphylococcus aureus decolonisation protocol in routine clinical practice' [Journal of Hospital Infection (2010) 93-98].

The Journal of hospital infection·2016
Same author

Altered hippocampal function in major depression despite intact structure and resting perfusion.

Psychological medicine·2016

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Primary transcripts of the neural cell adhesion molecule (NCAM) undergo extensive alternative splicing, producing over 100 mRNA variants.
  • These variants encode three major polypeptide isoforms (120, 140, and 180 kDa), differing by the inclusion of small exons at specific locations.
  • Understanding NCAM microsplicing patterns is crucial for elucidating its role in neural development and function.

Purpose of the Study:

  • To investigate the developmental regulation of NCAM microsplicing patterns.
  • To identify specific NCAM mRNA forms present during different stages of brain development.
  • To differentiate microsplicing regulation across major NCAM polypeptide isoforms.

Main Methods:

  • Analysis of NCAM mRNA populations from perinatal and adult rat brains, neuronal cultures, and glial cultures.

Related Experiment Videos

  • Quantification of alternatively spliced exons, including the VASE exon (30 bp) at the exon 7/8 junction and exons (3, 15, 18 bp) at the exon 12/13 junction.
  • Comparison of microsplicing patterns across different NCAM major size classes (120, 140, 180 kDa).

    • Main Results:

    • The VASE exon inclusion increased significantly with brain development, reaching approximately 50% in adult brains for all NCAM isoforms.
    • Microsplicing at the exon 12/13 junction was differentially regulated: smaller exons (3, 15, 18 bp) were abundant in 120 kDa NCAM mRNAs but rare in 140 and 180 kDa mRNAs after embryonic day 15.
    • Neuronal cultures showed similar exon 12/13 junction patterns to adult brain, while glial cultures exhibited higher levels of a 3 bp exon at this junction.

    Conclusions:

    • NCAM microsplicing is dynamically regulated during brain development, with distinct patterns for different exon junctions and NCAM isoforms.
    • The VASE exon is developmentally upregulated across all major NCAM isoforms.
    • Exon usage at the 12/13 junction is isoform-specific and cell-type influenced, suggesting distinct functional roles for different NCAM variants.