Related Experiment Video
Updated: Jul 12, 2026

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
Published on: January 7, 2018
Gastric and pancreatic lipase levels during a test meal in dogs
F Carrière1, R Laugier, J A Barrowman
1Biochemistry and Molecular Biology Center, National Center for Scientific Research, Marseilles, France.
Abstract:
The levels of gastric and pancreatic lipases in the duodenum and the jejunum were measured during the digestion of a test meal in dogs. Using both a specific enzymatic titration and an enzyme-linked immunosorbent assay, it is shown for the first time that gastric lipase remains active in the duodenal and jejunal contents. An experimental device was set up for measuring the secretions and the intestinal flows of lipases during the digestion of a liquid test meal. In a dog equipped with gastric and duodenal cannulae, the secretion of gastric lipase was stimulated by food ingestion, reaching 3.0 +/- 0.3 mg/h (three times the basal secretion rate) during the 1st h of digestion. The total secretory outputs of gastric and pancreatic lipases recorded over a 3-h period of digestion were 7.2 +/- 1.2 mg and 18.7 +/- 1.2 mg, respectively.
Related Concept Videos
Lipid Digestion
Hormones Regulating Blood Glucose
In addition to accelerating glucose uptake and utilization, insulin has...
Serum Laboratory Studies, Stool Test, Breath Test
Acute Pancreatitis I: Introduction

