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Animal model for persistent tympanic membrane perforations

O Spandow1, S Hellström

  • 1Department of Otorhinolaryngology, University Hospital, Umeå, Sweden.

The Annals of Otology, Rhinology, and Laryngology
|June 1, 1993
PubMed
Summary

Hydrocortisone treatment created persistent tympanic membrane perforations in rats, hindering natural healing. Hyaluronan and wound stimulation accelerated closure of these chronic perforations.

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Area of Science:

  • Otolaryngology
  • Regenerative Medicine
  • Animal Models

Background:

  • Tympanic membrane (TM) perforations can impair hearing.
  • Developing chronic perforations poses a challenge for healing.
  • Hydrocortisone is explored for its potential to create persistent perforations.

Purpose of the Study:

  • To establish an animal model for chronic tympanic membrane perforations using topical hydrocortisone.
  • To investigate the histological characteristics of hydrocortisone-induced persistent perforations.
  • To evaluate interventions for promoting healing in these chronic perforations.

Main Methods:

  • Standardized tympanic membrane perforations were created in rats.
  • Hydrocortisone suspension was applied topically to perforation margins daily for 10 days.
  • Healing was monitored, and histological analysis was performed at 3 months.

Main Results:

  • Hydrocortisone treatment prevented closure of TM perforations, with all remaining open after 50 days.
  • Control perforations healed within 9-12 days.
  • Histological examination revealed thickened TMs with keratin and wax, and altered connective tissue in persistent perforations.

Conclusions:

  • Topical hydrocortisone effectively models persistent tympanic membrane perforations in rats.
  • Hyaluronan application and perforation border wounding accelerated healing in chronic perforations.
  • This model aids research into mechanisms and treatments for non-healing TM perforations.

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