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Related Experiment Videos

Structure of the human C6 gene

M J Hobart1, B Fernie, R G DiScipio

  • 1MIP Unit, MRC Centre, Cambridge, U.K.

Biochemistry
|June 22, 1993
PubMed
Summary

The study of complement system proteins C6-C9 reveals a complex evolutionary history. Their gene structures challenge previous assumptions about protein module evolution and gene complexity.

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The architectural transition of human complement component C9 to poly(C9).

Molecular immunology·1999

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Terminal complement components (C6-C9) are related mosaic proteins.
  • Mosaic proteins are typically composed of homologous modules.
  • Gene structure of mosaic proteins often involves introns and partial gene duplication.

Purpose of the Study:

  • To investigate the gene structure and evolutionary history of complement system components C6-C9.
  • To examine intron/exon boundaries in C6 and C7 genes.
  • To compare findings with existing data for C9.

Main Methods:

  • Analysis of intron/exon boundaries for 17 of 18 exons in C6.
  • Examination of intron/exon boundaries for five exons in C7.
  • Integration of published data for C9 exon/intron structures.

Main Results:

  • Only one protein element within C6-C9 followed the conventional gene structure pattern.
  • Intron/exon boundary analysis revealed deviations from expected patterns.
  • The gene structures suggest a non-linear evolutionary path.

Conclusions:

  • The evolutionary history of terminal complement component genes is more complex than previously thought.
  • Protein module discovery does not reliably predict gene structure in these components.
  • These genes likely did not evolve from simple to complex in a straightforward manner.

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